Phytochemical activities in milkvetch (Astragalus psilocentros Fisch.) and japanese shikimi (Skimmia laureola dc. Sieb.) Plants from Sudhan Galli, district Bagh, Azad Kashmir
Keywords:
Antioxidant activities, milkvetch, flavonoids, medicinal plants, phenolic contentAbstract
In the current study, medicinal plants from Bagh district were collected and screened for the estimation of total flavonoids content, total phenolic content and antioxidant activities. The antioxidant activity was measured by 2-2 Diphenyl-1-picryi-hydrazyl (DPPH). A. psilocentros exhibited 52.2 ± 0.03 mg/100 g of alkaloids in leaf, 23.1 ± 0.07 mg/100 g in stem and 19.75 ± 0.14 mg/100 g in root. S. laureola exhibited 231.7 ± 0.9 mg/100g of alkaloids in leaf, 32.3±0.5 mg/100 g in stem and 15.34±0.7 mg/100 g in roots. Total phenolic content (TPC) ranged from 53.03 ± 0.13 to 44.01 ± 0.09 in A. psilocentros. In S. laureola, obtained range was from 71.31 ± 0.04 to 55.33 ± 0.06 mg GAE/g. The maximum content was found in stem 71.31 ± 0.04 mg GAE/g. Ethanol extract (EtE) of leaf, stem and root showed that the flavonoid content (TFC) of A. psilocentros and S. laureola extracts varied from 97.23 ± 0.11 to 49.14 ± 0.06 mg Rutin/g in A. psilocentros and 116.06 ± 0.03 to 85.24 ± 0.02 mg Rutin/g in S. laureola. In A. psilocentros and S. laureola, ethanolic extract of stem showed maximum (DPPH) scavenging activity (28±0.05 and 19±0.16 μg/mL) respectively. Above results revealed that ethanolic extract is reliable for electron donating capability and could work as free radical inhibitors, most likely chief oxidation inhibitors and utilizing the plant extracts in traditional medicines can be aided to cure most persistent human diseases.
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Copyright (c) 2023 Shameen Arif, Ali Akbar, Khawaja Shafique Ahmad, Zukhruf Tauqeer, Muhammad Taimur, Tehmina Zafar, Basharat Mahmood
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